LIFE SCIENCES, ANALYTICAL & MEDICAL TECHNOLOGIES
Equipment Applications for DNA Sequencing and PCR
Principle & OEM Spectroscopy Components
DNA sequencing determines the order of nucleotides within a DNA molecule. This method reveals valuable information about the role of inheritance and susceptibility to disease. It is employed in medical diagnosis and treatment through gene therapy.
Sanger Sequencing and Fluorescence Spectroscopy
Sanger sequencing is a DNA sequencing technique using the principle of Fluorescence spectroscopy. After the amplification step, PCR is halted by the addition of ddNTPs tagged with different fluorophores. The DNA fragments are arranged in size by capillary gel electrophoresis, passing in front a laser excitation. Specific fluorescence bands are emitted and the resulting chromatogram is processed for analysis.
Spectral Benches for DNA Sequencing
Fluorescence signals are weak. To guarantee adequate SNR and LOD levels, it is critical to select an optical bench with high throughput. Concave grating spectrometers, by their design, deliver the required ultimate performance. When configured with high QE, low read noise back-thinned CCD sensors, cooled or uncooled, these spectrometers deliver the best system combination.
tec5USA CGS and MCS Series of CCD spectrometers are the ideal candidates for high throughput DNA sequencing.
